| Visiting Scholar | |
| Office: | Biotech
Building 4119 |
| Telephone: | 518-276-2811 |
| Fax: | 518-276-2207 |
| E-mail: | leem2@rpi.edu |
| Mailing
Address: |
Dept. of Chemical
and Biological Engineering Rensselaer Polytechnic Institute Center for Biotechnology and Interdisciplinary Studies, Room 4211 110 8th Street Troy, NY 12180-3590 |
| Background | Research Area | Publications | Highlights | Honors | |
Education:
B.S.,
Chemical Engineering, Gyeong Sang
M.S.,
Chemical Engineering,
Ph.D.,
Chemical and Biomolecular Engineering, KAIST,
Post-doc.,
Chemical Engineering, Tokyo
Institute of Technology,
Post-doc.,
Chemical and Biological Engineering, Rensselaer
Polytechnic Institute (RPI),
Professional
Experience:
2004-present Senior Scientist, Solidus
Biosciences, Inc.,
2004-present Visiting
Scholar, RPI, Inc.,
2001-present Research
Consultant,
NANONIX,
2001-2001 Chief Researcher,
NANONIX,
1999-2001 JSPS
Researcher, Tokyo Institute of Technology,
Current Research Interests:
· Biocatalysis
in Microarray Biochips for In Vitro Drug Metabolism and
High-throughput
Drug Screening
- In situ drug metabolite synthesis and in vitro toxicology assay on protein/cell chip
- Fabrication of microscale protein/cell chip by protein and cell immobilization
- Cell-based high-throughput assay development
- Assay miniaturization from microliter (mL) to nanoliter (nL) scale
· Biocatalysis
in Microfluidic Biochips for In Vitro Metabolic Engineering
- Fabrication of microfluidic protein/cell chip: surface chemistries on the microchannel for protein and cell immobilization
- Continuous drug metabolite synthesis by immobilized P450 and cytotoxicity assay in microfluidic chip
· Biocatalysis
in Extreme Conditions Enabling New Chemistries and Functionalities for
Development of Drugs
-
Surface-active enzymes and their unique interfacial reactions
-
Non-aqueous enzyme reactions
· Chemical Surface Modification of Nanoparticle
and Self-assembly for Nanobiotechnology
- Patterned 3D
protein
nanostructures with organic/inorganic
nanoparticles
-
Self-assembly of surface-active materials
Refereed Journal Articles:
34. Lee, M.Y., Hong, K.J. and Kajiuchi, T., Enzyme attached on polymeric micelles as a nanoscale reactor (In preparation, 2007)
33. Hong, K.J., Lee, M.Y., Kim, K.A. Shinya, Y. and Kajiuchi, T., Surface-active peroxidase and its characteristics of interfacial reaction with phenolic compounds (In preparation, 2007)
32.
Kwon, S.J.*, Lee, M.Y.*, Ku,
B.S. and Dordick,
J.S., In vitro metabolic
pathway microarray: A new paradigm
to discover novel natural product analogs (In preparation, 2006) [*Equally
contributed author]
31. Lee, M.Y., Ramasubramanian, A.K., Hogg, M., Sukumaran, S., Clark D.S. and Dordick, J.S., Three-dimensional cellular array chip for microscale toxicology assay (In preparation, 2006)
30. Lee, M.Y. and Dordick, J.S., High-throughput human metabolism and toxicity analysis, Current Opinion in Biotechnology, 17, 619-627 (2006)
29. Lee, M.Y.,
Clark D.S. and Dordick, J.S., Human
P450 microarrays for in Vitro metabolite
toxicity analysis, Journal
of the Association for Laboratory
Automation (In press,
2006) [Invited
paper]
28.
Lee, M.Y., Park, C.B., Clark, D.S.
and Dordick, J.S., Metabolizing enzyme toxicology assay chip
(MetaChip) for high-throughput microscale toxicity analyses, Proceedings of the National Academy of Sciences of the United States of America
(PNAS), 102(4), 983-987
(2005)
[Featured as cover article]
27. Lee, M.Y., Hong, K.J., Kajiuchi, T. and Yang, J.W., Synthesis of chitosan-based polymeric surfactants and their adsorption properties for heavy metal and fatty acid, International Journal of Biological Macromolecules, 36(3), 152-158 (2005)
26. Lee, M.Y., Hong, K.J., Sudo, S. and Kajiuchi, T., Adsorption of chromium by chitosan-based polymeric surfactants, Journal of Applied Polymer Science, 96(1), 44-50 (2005)
25. Lee, M.Y., Hong, K.J., Kajiuchi, T. and Yang, J.W., Determination of the efficiency and removal mechanism of cobalt by crab shell particles, Journal of Chemical Technology & Biotechnology, 79(12), 1388-1394 (2004)
24. Shin-ya, Y., Yoshizawa, T., Hong, K.J., Lee, M.Y. and Kajiuchi, T., Log-normal distribution for a description of the molecular weight distribution of N-acetylated chitosans depolymerized with hydrolytic enzyme, Polymer International, 52(5), 838-842 (2003)
23. Lee, M.Y., Srinivasan, A., Ku, B.S. and Dordick, J.S., Multienzyme catalysis in microfluidic biochips, Biotechnology and Bioengineering, 83(1), 20-28 (2003)
22. Srinivasan, A., Wu, X.Q., Lee, M.Y. and Dordick, J.S., Microfluidic peroxidase biochip for polyphenol synthesis, Biotechnology and Bioengineering, 81(5), 563-569 (2003)
21. Lee, M.Y. and Dordick, J.S., Enzyme activation for nonaqueous media, Current Opinion in Biotechnology, 13, 376-384 (2002)
20. Kang, H.A., Jeon, G.J., Lee, M.Y. and Yang, J.W., Effectiveness test of alginate-derived polymeric surfactants, Journal of Chemical Technology and Biotechnology, 77(2), 205-210 (2002)
19. Kang, H.A., Shin, M.S., Lee, M.Y. and Yang, J.W., Characteristics of newly synthesized alginate-derived surfactants, Preprints of Extended Abstracts Presented at the ACS National Meeting, Division of Environmental Chemistry, 41(2), 238-242 (2001)
18. Shin-Ya, Y., Lee, M.Y., Hinode, H. and Kajiuchi, T., Effects of N-acetylation degree on N-acetylated chitosan hydrolysis with commercially available and modified pectinases, Biochemical Engineering Journal, 7(1), 85-88 (2001)
17. Lee, M.Y. and Yang, J.W., Biosorption of cobalt by methanotrophic biomass, Journal of Korean Society of Environmental Engineering, 22(12), 2163-2173 (2001)
16. Lee, M.Y. and Yang, J.W., Biosorption of lead and cobalt by Absidia coerulea and Thraustochitrium sp., Journal of Korean Society of Environmental Engineering, 22(12), 2149-2161 (2001)
15.
Kang, H.A., Shin, M.S., Lee, M.Y., Jeon, G.J. and Yang,
J.W., Synthesis of alginate-derived polymeric surfactants, Korean
Journal of Biotechnology
and Bioengineering, 15(4), 375-379 (2000)
14. Lee, M.Y. and Yang, J.W., Characteristics of lead removal by methanotrophic biomass, Korean Journal of Biotechnology and Bioengineering, 15(5), 444-451 (2000)
13. Lee, M.Y., Kang, H.A. and Yang, J.W., Characteristics of lead removal in a fixed-bed column packed with activated carbon and crab shell, Journal of Korean Society of Environmental Engineering, 22(10), 1747-1756 (2000)
12. Lee, M.Y., Cho, H.J. and Yang, J.W., Factors of trichloroethylene degradation by methanotrophic consortium biofilm reactor (MCBR), Journal of Korean Society of Environmental Engineering, 22(6), 991-1000 (2000)
11. Lee, M.Y. and Yang, J.W., Initial condition of methanotrophic consortium biofilm reactor (MCBR) for trichloroethylene degradation, Journal of Korean Society of Environmental Engineering, 22(5), 971-980 (2000)
10. Lee, M.Y., Var, F., Shin-Ya, Y., Kajiuchi, T. and Yang, J.W., Optimum conditions for the precipitation of chitosan oligomer with DP 5-7 in the concentrated hydrochloric acid at low temperature, Process Biochemistry, 34(1), 493-500 (1999)
9. Lee, S.H., Shon, J.S., Chung, H.S., Lee, M.Y. and Yang, J.W., Effect of chemical modification of carboxyl groups in apple residues on metal ion binding, Korean Journal of Chemical Engineering, 16(5), 576-580 (1999)
8. Shin, H.J., Lee, M.Y. and Yang, J.W., Biodegradation of trichloroethylene by phenol-degrading Pseudomonas putida, Journal of Microbiology Biotechnology, 8(2), 185-187 (1998)
7. Lee, M.Y., Lee, S.H., Shin, H.J., Kajiuchi, T. and Yang, J.W., Characteristics of lead removal by crab shell particles, Process Biochemistry, 33(7), 749-753 (1998)
6. Lee, M.Y., Shin, H.J., Lee, S.H., Park, J.M. and Yang, J.W., Removal of lead in a fixed-bed column packed with activated carbon and crab shell, Separation Science and Technology, 33(7), 1043-1056 (1998)
5. Lee, S.H., Jung, C.H., Chung, H.S., Lee, M.Y. and Yang, J.W., Removal of heavy metals from aqueous solution by apple residues, Process Biochemistry, 33(2), 205-211 (1998)
4. Lee, M.Y., Shin, H.J., Yeom, S.P. and Yang, J.W., Biodegradation of trichloroethylene by a methanotrophic consortium, Journal of Korean Society of Environmental Engineering, 20(9), 1207-1217 (1998)
3. Lee, M.Y., Shin, H.J., Yeom, S.P. and Yang, J.W., Isolation and cultivation of methanotrophic consortium for trichloroethylene degradation, Korean Journal of Biotechnology and Bioengineering, 13(5), 483-490 (1998)
2. Lee, M.Y., Park, J.M. and Yang, J.W., Micro precipitation of lead on the surface of crab shell particles, Process Biochemistry, 32(8), 671-677 (1997)
1. Lee, M.Y. and Yang, J.W., Bioremediation of groundwater contaminated by halogenated aliphatic compounds, The Microorganisms and Industry, 21(4), 409-417 (1995)
Patents:
5. Lee, M.Y., Ramasubramanian, A.K., Clark D.S. and Dordick, J.S., Three-dimensional cellular array chip for microscale toxicology assay, US Patent submitted (2005)
4.
Dordick, J.S., Lee, M.Y., Srinivasan, A., and Ku, B.S.,
Enzyme immobilization for electroosmotic flow, US Patent 10/351,976 (In
pending, 2003)
3. Lee, M.Y., Synthesis of surface-active enzyme and its applications for interfacial reaction, Korea Patent 10-2002-20609 (In pending, 2002)
2. Lee, M.Y., Choi, K.Y. and Yang, J.W., Anti-microbial activity of chitin/chitosan oligomers and surfactants derived from chitin/chitosan and their applications, Korea Patent (1999)
1. Lee, M.Y., Choi, K.Y. and Yang, J.W., Chitin/chitosan-based surfactant and manufacturing method therefor, Korea Patent 0302158 (1998)
Research Experience:
· In Situ Drug Metabolism and In Vitro Cytotoxicity Assay on P450 Microarray Biochip Coupled with Cell Chip Containing Cancer Cell Lines
- Sol-gel and hydrogel immobilization of enzymes on microarray biochip for high- throughput drug screening; cytochrome P450 isozymes (CYP3A4, 2B6, 1A2, etc.) and other drug-metabolizing enzymes
- Optimization of sol-gel formulation using design of experiments (MINITAB)
- Chemical surface modification of glass slide for enzyme/cell immobilization
- Evaluation of enzyme kinetics using fluorogenic substrates
- Mammalian cell culture; human caner cell lines including MCF7, Hep3B, A293, etc.
- Live cell immobilization on the surface of micro-patterns
- Microimprinting techniques to deliver drug metabolites to the cancer cell lines
- Cytotoxicity test on the P450 chip; MTT, live/dead cell staining
- Computer programming for microarrayer operation
· Multi-enzyme Immobilization on a Microfluidic Biochip for In Vitro Metabolic Engineering
- Fabrication of microfluidic devices using photolithography, wet etching and plasma- thermo; glass & PDMS chip, etc
- Chemical surface modification of the microchannel; attachment of -NH2/-COOH group
- Immobilization of enzymes in the microchannel for high-throughput biochips; polymaleic anhydride, glutaraldehyde, photo-immobilization, etc
- Transportation of biological molecules in the microchannel by electro-osmotic flow, centrifugal force, micro-syringe pump, etc.
- Multi-enzyme reaction on the chip; peroxidase, lipase, glucose oxidase, P450, etc
- Non-aqueous enzyme reaction using ionic solvents in microfluidic system; lipase
- Modeling microscale enzyme reactor
· Surface-active Enzymes as Nanoscale Reactor and Their Interfacial Reactions
- Synthesis of surface-active enzymes by attaching amphiphilic polymers in O/W, W/O emulsion, reverse micelle; lipase, peroxidase, pectinase, etc
- Optimization of synthetic conditions of surface-active enzymes
- Synthesis of phenolic polymers with surface-active peroxidase in aqueous solution
- Polylactic acid synthesis with surface-active lipase in aqueous-organic biphasic system
- Hydrolysis of lipophilic substrates with surface-active lipase in aqueous solution
· Nanoparticle Self-assembly and Surface Modification
- Patterned 3D protein nanostructures; attachment of enzymes on gold nanoparticle and its self-assembly
- Chemical modification of nanomaterial surface; silicon nano-pillar, carbon nanotube, gold nanoparticle, silicon and glass surface, etc
- Protein thin
film for
THz spectroscopy
· Modification of Enzyme Surface for Nonaqueous Biotransformation
- Modification of enzyme surface with polymers (e.g., PEO, PMA and PEG) and surfactants (e.g., AOT, Brij, Tween, etc.)
· Chemical Synthesis of Functional Surfactant
- Synthesis of functional biopolymer-based surfactants; chitin/chitosan and alginate
- Anti-microbial surfactant for medical applications; chitosan-based surfactants
- Surfactants with different functional group for cosmetic applications; chitosan-based surfactants
· Micellar-enhanced Pollutant Removal, Surface Precipitation and Adsorption
- Synthesis of functional surfactant and adsorbent for soil remediation
- Micellar-enhanced ultrafiltration for the removal of pollutants; heavy metal, radionuclide, crude oil, etc
- Precipitation of heavy metal and radionuclide on the surface of biosorbents
· Biodegradation of Organic Pollutants using Microorganisms
- Isolation, fermentation and immobilization of methanotrophic bacteria for the degradation of toxic chlorinated organic compound
- Design and
construction
of pilot plant-scale continuous bioreactor (100 L)
· A Successful Fellow
of Korea-Japan Researchers' Friendship Award, Korea-Japan Cooperation Foundation
for
Industry and Technology (KJF), 1996
· Short-Term Student Exchange
Promotion
Program (Peace and Friendship Scholarship), Association of
International
Education,
· Postdoctoral
Fellowship Program for Successful Foreign Researcher,
· Postdoctoral
Fellowship
· Named “People
glorifying