I joined the Dordick lab in March 2020, right after I finished my Ph.D degree in Korea. My previous work focused on the enzyme immobilization and stabilization via nanobiocatalytic approaches with various kinds of nanostructured materials such as carbon nanotubes and mesoporous materials. I applied these nanobiocatalytic materials for the enzymatic microbial decontamination such as antifouling and biocidal approach, as well as glucose oxidase-based biofuel cell application and enzymatic CO2 conversion and utilization (CCU).
2020, Ph.D. in Chemical and Biological Engineering, Korea University, Republic of Korea (Prof. Jungbae Kim)
Dissertation: Development of Nanobiocatalytic Materials using Glucose Oxidase for Biofuel Cell and Antibacterial Applications
2010, B.S. in Chemical and Biological Engineering, Korea University, Republic of Korea (Prof. Jungbae Kim)
My current research focuses on the cell wall binding domain (CBD) study for the detection of pathogenic bacteria. I have designed and constructed the recombinant CBD proteins fused with binder molecule and/or fluorescent protein and applied them to the spectroscopy-based and paper-based detection platforms suitable for target bacterial detection. I am also interested in CRISPR-based detection of target proteins.
1. Inseon Lee, Seok-Joon Kwon, Mirco Sorci, Peter S. Heeger, and Jonathan S. Dordick, Highly Sensitive Immuno-CRISPR Assay for CXCL9 Detection, Anal. Chem. 93, 16528–16534 (2021).
2. Inseon Lee, Hong Jae Cheon, Manab Deb Adhikari, Tai Duc Tran, Kyung-Min Yeon, Moon Il Kim and Jungbae Kim, “Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent”, Int. J. Biol. Macromol. 155, 1520-1531 (2020).
3. Domyoung Kim, Seok-Joon Kwon, Jessica Sauve, Keith Fraser, Leighann Kemp, Inseon Lee. Jahyun Nam, Jungbae Kim and Jonathan Dordick, “Modular assembly of unique chimeric lytic enzymes on a protein scaffold possessing anti-staphylococcal activity”, Biomacromolecules 20, 4035-4043 (2019).
4. Kyung-Min Yeon, Jisung You, Manab Deb Adhikari, Sung-Gil Hong, Inseon Lee, Han Sol Kim, Seok-Joon Kwon, Moon Il Kim, Warayuth Sajomsang, Jonathan S. Dordick and Jungbae Kim, “Enzyme-immobilized chitosan nanoparticle as environmentally-friendly and highly effective antimicrobial agents”, Biomacromolecules, 20, 2477-2485 (2019) [cover image article].
5. Seok-Joon Kwon, Domyoung Kim, Inseon Lee, Jahyun Nam, Jungbae Kim and Jonathan S. Dordick, “Sensitive multiplex detection of whole bacteria using self-assembled cell binding domain complexes”, Anal. Chim. Acta., 1030, 156-165 (2018).
6. Domyoung Kim, Seok-Joon Kwon, Jessica Sauve, Inseon Lee, Jahyun Nam, Jungbae Kim and Jonathan S. Dordick, “Selective killing of pathogenic bacteria by antimicrobial silver nanoparticle - cell wall binding domain (CBD) conjugates”, ACS Appl. Mater. Interfaces., 10, 13317-13324 (2018).
7. Tae Hee Kim, Inseon Lee, Kyung-Min Yeon and Jungbae Kim, “Biocatalytic membrane with acylase stabilized on intact carbon nanotubes for effective antifouling via quorum quenching”, J. Membr. Sci., 554, 357-365 (2018).
8. Jeongjoon Lee, Inseon Lee, Jahyun Nam, Dong Soo Hwang, Kyung-Min Yeon and Jungbae Kim, “Immobilization and Stabilization of Acylase on Carboxylated Polyaniline Nanofibers for Highly Effective Antifouling Application via Quorum Quenching”, ACS Appl. Mater. Interfaces., 9, 15424-15432 (2017).
9. Seok-Joon Kwon, Domyoung Kim, Inseon Lee, Jungbae Kim and Jonathan S. Dordick, “In vitro gene expression-coupled bacterial cell chip for screening species-specific antimicrobial enzymes”, Biotechnol. Bioeng., 114, 1648-1657 (2017).
10.Byoung Chan Kim, Inseon Lee, Seok-Joon Kwon, Youngho Wee, Ki Young Kwon, Chulmin Jeon, Hyo Jin An, Hee-Tae Jung, Su Ha, Jonathan S. Dordick and Jungbae Kim, “Fabrication of enzyme-based coatings on intact multi-walled carbon nanotubes as highly effective electrodes in biofuel cells”, Sci. Rep. 7, 40202 (2017) [equally contributed author].
11.Gudi Satheesh Kumar, Youngho Wee, Inseon Lee, Ho Jin Sun, Xueyan Zhao, Shunxiang Xia, Ping Wang and Jungbae Kim, “Stabilized glycerol dehydrogenase for the conversion of glycerol to dihydroxyacetone”, Chem. Eng. J., 276, 283-288 (2015).
12.Kie Moon Woo, Inseon Lee, Sung-Gil Hong, Sunhyung An, Jinwoo Lee, Euichaul Oh and Jungbae Kim, “Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion”, Chem. Eng. J., 276, 232-239 (2015) [equally contributed author].
13.Young Hyun Kim, Inseon Lee, Sung Hee Choi, Ok Kyung Lee, Jungbae Kim, and Eun Yeol Lee, "Nanoimmobilization of marine epoxide hydrolase of Mugil cephalus for repetitive enantioselective resolution of racemic styrene oxide in aqueous buffer", J. Nanosci. Nanotechnol., 13, 2266-2271 (2013) [equally contributed author].
14.Young Hyun Kim, Inseon Lee, Sung Hee Choi, Ok Kyung Lee, Jongmin Sim, Jinwoo Lee, Jungbae Kim, and Eun Yeol Lee, "Enhanced stability and reusability of marine epoxide hydrolase shipped-in-a-bottled into magnetically-separable mesoporous silica", J. Mol. Catal. B: Enzym., 89, 48-51 (2013) [equally contributed author].
15.Mike Fischback, Ki Young Kwon, Inseon Lee, Su Jeong Shin, Hyun Gyu Park, Byoung Chan Kim, Yongchai Kwon, Hee-Tae Jung, Jungbae Kim and Su Ha, "Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications", Biotechnol. Bioeng., 109, 318-324 (2012).
16.Hyeongseok Kim, Inseon Lee, Yongchai Kwon, Byoung Chan Kim, Su Ha, Jung-heon Lee, and Jungbae Kim, "Immobilization of glucose oxidase into polyaniline nanofiber matrix for biofuel cell applications", Biosens. Bioelectron., 26, 3908-3913 (2011) [equally contributed author].